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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which leads to chromosome instability, and therefore may well lead to tumorigenesis. This protein was located to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two further splice variants have been described but in depth nucleotide sequence info will not be but accessible.
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Blocking peptides are peptides that bind specially for the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized because of the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible final results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E forms a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is particularly degraded as cells progress through click here S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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